Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHATSLPPELHQWVTRHLTGMDTAAVTDASWPRGASRVWRVRTDH-GEVFVKLYPST---EKYEREVQGCEHATRALAADEAPRLLASDPD---RPA----VVLSALPGRVVRGLPLEQAEEQRVHRLAGGLLRRWHDTPEPIGDREHQRIRASVTAQADEAAVILERLGDQL--APAERNLVRDAAHDLPDLARALPLAYRHGDYSPRNWLWDEAAGHHSLIDFEESAPGLAVEDLVWLCGAAWPTRPDLRDAFLTGYGRLLS---STEQRALVLLTARLGVSYLSTGLTKADTVLIDRGRTVLQHMVRAPR 5IGI Chain:A ((8-300)) ------DTSQLYALAAR-HGLKLHGPLTVNELGL-DYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRL-PFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV-VPQDSEVFAESFATALAALHAVPISAAVDAGMLI-RTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDD--SSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF---GEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLAAA-----

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1267 -18370 -14.50 -66.56 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -14.50 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: