Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence VCRALIP-ELQDADDSDFDVCRINGGITNISDGRFLSFAVAKVSKKDQSVVVRVFGPATEGVIDRDREIQAT----------CHLSRAGFCPELKGVFKNGIIQTFVTARTLTPEDFLDDAVVAKVAKELRRLHQQEVPGEKE-SMVWTEINRYFELASAVTKFESPEDQRKLEAVSFDELRQEINTLKEIGARLKGPVVYAHNDLLPGNVMV----DAQGDKCYYFIDFEYSGYNYRGFDIGTHFNE---------YAGFDCDFCAYPSKDRQLNFLRHYLRP--DDPEKATHEE----LEELFVEANFYALAAHITWSAWAIVQATSSAAIDFDYMSYFFKRMKMYREQKAKFLPVVRGFLAGKSK 2I7Q Chain:A ((40-400)) -CKEFLPGAWRGLREDEFHISVI------MLFQCSLPDTTATLGDEPRKVLLRLY-----------------EAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRI-KFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKIS-SIEFGYMDYAQARFDAYFHQKRKL-------------

General information: TITO was launched using: RESULT: Template: 2I7Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1238 14500 11.71 48.66 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 11.71 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_2I7Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2i7q-query.scw PDB file : Tito_Scwrl_2I7Q.pdb: