Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTVAAPAPALAEVACFSRDLCKE-LVKEWSSLDSSCFSISTVSGGITNMLLKVSVKEGS----DSESSVTVRLYGPNTDLVIDRERELQGSGLGYLEYLRMEWFRHSSMLGHYHLQVYTQRLL--FCTHRCMETGEPAINASANNMKEPRIAAEIAKQLQKFHQVDIPGSKEPQ-LWNDIFKFLKKASVLKFEDNEKQKRYETISFREIQDEVKELKDLSDLLHAPVVFAHNDLLSGNLML-----NDLEEKLYFIDFEYGSYSYRGFDIANHFNE---------YAGFECDYNLYPDKDVQYHFFRNYLSDRPSELKNRLFASICEKRNVGYLAFVQYKVQEQDLDDLYTETNTFRLASHLYWALWALIQARVSPIDFDYLGYFFLRRHVVPNRKLYNL 2CKO Chain:A ((46-373)) ---------------------CKEFLPGAWRGLREDEFHISVIRGG-SNMLFQCSLPDTTATLGDEPRKVLLRLYGAI----------------MVLESVMFAILAERS-LGPKLYGIFPQGRLEQFIPSRRLDTEELSL---------PDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFEN---LSTEEKSIIK--------------EEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDY----------------

General information: TITO was launched using: RESULT: Template: 2CKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1177 23018 19.56 77.24 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 19.56 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.351

(partial model without unconserved sides chains): PDB file : Tito_2CKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2cko-query.scw PDB file : Tito_Scwrl_2CKO.pdb: