Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELEPLLDSLAVLAGQEREVTLLPGGLTNANYRV----TTAT-----RDVVVRVASASSGLLSIDRDQEVHNSRAA----------AKAGVAPEVVEHRPGLLVVQFVP------DALTYGD--SDLAANLAGVADVVRRLHEGPAFA-GRFDMFDIQREYLAIMHTHRFRMPRGYLALAP----AAERMEAALRVHPEPRVPCHNDLLAANFL-------QDAQRLWIVDYEYSGMNEASFELGNIVQE------------------AHLTPEHLAELVTAYV-----------GHEDPVRTARAAL----WGIASAWAWTLWGTIQAGASDLDFDFWEWAMDKMDRAAQAFAEPGFDELLDTVGSPR 3ZM9 Chain:A ((36-372)) ----------------EFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLY----------------GAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKY--LKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFD----------------------

General information: TITO was launched using: RESULT: Template: 3ZM9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 809 -1050 -1.30 -4.35 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -1.30 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_3ZM9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3zm9-query.scw PDB file : Tito_Scwrl_3ZM9.pdb: