Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------HGGQTFHNELRCIAEFYEHGDISNYFC-HPK--SDVKPG-------G-----VIKCVYRDHPIVVKTDPGCNWKSTAG---------- 1PKL Chain:A ((89-194)) EIRTGQFVGGDAVMERGATC--YVTTDPAFADKGTKDKFYIDYQNLSKVVRPGNYIYIDDGILILQVQSHE-DEQTLECTVTNSHTISDRRGVNLPGCDVDL

General information: TITO was launched using: RESULT: Template: 1PKL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 164 10058 61.33 167.63 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 61.33 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_1PKL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1pkl-query.scw PDB file : Tito_Scwrl_1PKL.pdb: