Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENHEIRDRVESVYGHS-VSGIRDVSSN-QKQTYVLDTSDGEVVYQRCESPQ-FRESFRCEVLLLEQMRDV-RTPVPKVLWRELEN---T-PEAVISSAPGREVSEFY-GEQNFEQAGDVVKQAGTTLSQIHEKKFNHSGKLSTQGGLELKATSWEDILAEIFGDYIHQLQGSRFEDSITDAVALFDNGLDLVD--EHGSCLVHYDVAPDNVFWEN--GNISSVIDFGRAMSGDPAFDLAYAQVQFVLAFYDGREAEQLNRLLFGSYH-TESLRGEWHKRMWFYRKLFIVEAMKSFESWTEGQGMSEEEKQESAEWNRDQLSQAAAELR 3N4U Chain:A ((7-276)) ---DQVEKAIEQLYPDFTINTIEISGEGNDCIAYEI----NRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLV--------LDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDD-EE-----YGMEFVSKILNHYKHKDIP--TVLEKYRMKEKYWSFEKI------------------------------------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1098 61194 55.73 239.04 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 55.73 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: