Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDMKLSDSKTELARRHNKVVYQDG----N-RVVKVFNDQKPASDVFNEALNTARIMGT-DIKVSEVLEVSRIEGGEWNGSWA----IANSYIPGRTLRDIASEVSYPDKNNPDLDKLQEFTEKMVDLQISVQNVKAPLL----------NRQKDKLARMVNSVKA---IDPSTRYDLELRVDG---LHNIGAVCHGDFVPSNIIVADD-GELYICDWAHVTSGDPVVDAAQTYLLLKLRHPIWAESYLELYTRKADVAK----QKIHYWVPVVAAAELARGRKRDEDFLMAQVNAGDFQ 5IGI Chain:A ((26-282)) -------PLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAEL----VAYPMLEDSTA-VIQPGSSTPDWVV-----------PQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF--G-EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFAL-----------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 920 5327 5.79 23.67 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 5.79 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: