Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGKEVNMKNIEKEILKRINELYPLD-FINITP---VTNEMYQCLAKQGNYFVRITNYKTYEEQLEEVKYTDFLYKEGLGVPPIIPSLTGNLVEKITLNRELFTVLYKAAPGIHLPKSEWNSNVFKKLGQQIGKLHRISKNFESIEPV-IHINDWYENEEYNFLHYIPIEETTIRKIASDVITSIQELSKSPSNYGLIHGDLWLENILVENDLNLTMLDFQDCEKHFYIFDLAVPIYSAIEYSFVGNGNIVDYENSIIKAIVEGYKEEHDLPKEMLDQLPLFIKLKEIFEYSLMHMYWDKEELTEEQVRIMNLYRMKIENNHSFINI 2PPQ Chain:A ((5-286)) -------TDITEDELRNFL-TQYDVGSLTSYKGIAE--NSNFLLHTTKDPLILTLYEK--KNDLPFFLGLMQHLAAKGLSCPLPLPRKDGELLGEL---SGRPAALISFLEGMWLRKP--EAKHCREVGKALAAMHLASEGFEIKRPNALSVDGWKVLW-DKSEERADEVEKGLREEIRPEIDYLAAHWPKDLPAGVIHADLFQDNVFFLGDELSGLIDFYFACNDLLAYDVSICLNAWCFEK---D---GAYNVTKGKALLEGYQSVRPLSEAELEALPLLSRGSALRFFLTRLYDWLT---------------------------

General information: TITO was launched using: RESULT: Template: 2PPQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1281 10 0.01 0.04 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 0.01 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.343

(partial model without unconserved sides chains): PDB file : Tito_2PPQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ppq-query.scw PDB file : Tito_Scwrl_2PPQ.pdb: