Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDMKLSDSKTELARRHNKVVYQDG----N-RVVKVFNDQKPASDVFNEALNTARIMGT-DIKVSEVLEVSRIEGGEWNGSWA----IANSYIPGRTLRDIASEVSYPDKNNPDLDKLQEFTEKMVDLQISVQNVKAPLL----------NRQKDKLARMVNSVKA---IDPSTRYDLELRVDG---LHNIGAVCHGDFVPSNIIVADD-GELYICDWAHVTSGDPVVDAAQTYLLLKLRHPIWAESYLELYTRKADVAK----QKIHYWVPVVAAAELARGRKRDEDFLMAQVNAGDFQ 5IGH Chain:A ((26-282)) -------PLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAEL----VAYPMLEDSTAMVIQPGSSTPDWVV-----------PQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF--G-EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFAL-----------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 889 8587 9.66 38.00 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 9.66 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: