Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVGETVDRIVADALEAEVRSRYRPRSGSVAETVVCRLETGDGDGDEPPSRVVCKRGGASVWTGDVIEPLVLEYMTAATDLPVPRVLASGSFADGSNDPLERWAIYEFREGANPGPRYHKLEPTVRRRLVADAGELLGRLHGTSA------LAFDRVGGLARGADDRSLRLREPDGWHAVDPGWPLTRVPVPLAGDE-DCRPVLTHGDYQPSNLLVDDDGAVTAVLDWGNAHVTHAEYALARAEARFVDVYAGRLPRAERKQLQELFRRTYANCAPLETGFDRRAPVYKLLWVAQSGANYLRIAREPRGRRQLRTQVRRLVE 1J7L Chain:A ((2-264)) ----AKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLV------GENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDG------WSNLLMSEADGVLCSEE--YEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKD-GKVSGFIDLGRSGRADKWYDIAFCVRSIREDIG---EEQYVELFFDLLGIKPD---------WEKIKYYILLDELF---------------------------

General information: TITO was launched using: RESULT: Template: 1J7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1192 -18507 -15.53 -72.29 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -15.53 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_1J7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1j7l-query.scw PDB file : Tito_Scwrl_1J7L.pdb: