Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTELHSLVFDTLSHDQQVARLHELARHALQQWDGEF-ADIELVKF-RENAVFSARRHDGQRVALRIHRNGYHSQAALRSELQWMEALADA-GITVPPIIRAQNQSHLVEVTHQAIGEPRHIDMLAWLPGATAGTSENGVQADTEIDFLFSEAGAIAARIHLHSAHWQQPDEFVRHAWD--EEGLIGANPFWGRFWELEQLSDEQRDLLQQARRTARKDLRQYGRHLGNFGMIHADLVPENLLIE-G-PRLRLIDFDDAGFGWHMFELATALYFCLDDPRYEQIKVALLDGYNAVKPLTEAD-RKTLALFLMLRGTTYLGWIHTRQGTPTAIEMAPMLIERACLLAREYLQV 5IGI Chain:A ((10-280)) ----------------------SQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAE-VSAKVEPEARVLAMLKNRLPFAVPDWRVANA-ELVA-YP-----MLEDSTA-VIQPGSSTPDWVV----PQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRRE-----FV-VN----DKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG-----EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALF-------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1192 -38574 -32.36 -147.23 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -32.36 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.319

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: