Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSHIQRETSCSRPRLNSNLDADLYGYRWARDNVGQSGATIYRLYGKPNAPELFLKHG----KGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAW--LLTTAIPGKTAFQVLEEYPD--SGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPF------SPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQ--KRLFQKYGIDNPDMNKLQFHLMLDEFF 3TM0 Chain:A ((1-263)) ----------AKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENEN--LYLKMTDSRYKGTTY-DVEREKDMMLWLEGKLPVPKVLHFERH--DGWSNLLMSEADGVLCS---EEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADV-----DCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI-KPDWEKIKYYILLDELF

General information: TITO was launched using: RESULT: Template: 3TM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1115 9208 8.26 37.28 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 8.26 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_3TM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tm0-query.scw PDB file : Tito_Scwrl_3TM0.pdb: