Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIEINGKWIFRFPRNPISKENTKERLDFLISFSKVSSLKIPVPKYV---------ENNFIGYEKIQGSHPRPTNIKNLAELDKLKIAKQLGLFLKDLH--NFKSKKINFDTGYLIMRKGDYKTCPKEITKYLNADEIKNLETKLKAIANNPLNFKKPTSIIHGDFYFNNIIWNPDKKVITGVIDWANLGLGTPAMDFIMLADFNKNTNDKFLKDILKYYGAKGDDLFFQIKENAIIDAMNWFWSYYKEKNPKGVARMIKKLKKLLAE 4DFB Chain:A ((59-276)) ---EINRDFIFKFPKHSRGSTNLFNEVNILKRIH--NKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLME--EEYGMEFVSKILNHYKHK---------------------------------------------

General information: TITO was launched using: RESULT: Template: 4DFB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 795 44838 56.40 218.72 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 56.40 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_4DFB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4dfb-query.scw PDB file : Tito_Scwrl_4DFB.pdb: