Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDGPGSDPIDRALAAAFPDREVDRLTGVGPSWNGANETVD-VAFADGGRAFLKVALADESNRLARARAVLRYVGAHGQLPVPEVLAADPDGDPAYLATAPAPGRGLLDVYEAASDDERERLLRRVGRVLAALHADRFASHGEIVGAGTERGSVAEGAATAGPDAQTGLAIEFAPWTD-VLLATVERTREIGTSERLADHYDAVVDCVRANRDLLDDAPAALLHGDVTKPNLFVTAEGDGADATDGPGVAAIDWELSHVGDPARDLVRAEDQLLNGFDSTGPERFAAALREGYRERAGGLPAGFERRRPVYEVVRMLGRSGFVDQWVPYLDEPLDALVDRADAELRARLDAV 3CSV Chain:A ((4-274)) ---SREDEIRDFLAT-H-GYADWNRTP----------RYQRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNL-DISAPEIYAEEHA--RGLLLIEDLGDALFTEVINNDP-AQEMPLYRAAVDLLIHLHDAQTPELARLDPE------------------------TLSEMTRLAFSEYRYAILG----DAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPER-----EGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDV----PAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRIL--------------------------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1175 21238 18.07 81.37 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 18.07 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: