Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDTDSLKNARFYATKTEPITDVDIVAEAPLERPELIKGKDAPGILTFARVH-REGGSDLYQLVVDKQ-G--R-------------DILGEEETARAAGKQLSNGYAV-------GSGVLHKLTDPALPP-VETVRAV-GEQSNTSWIYNDRVIVKYFRRLTPTPNPEIELLEELTAAGCTHSAPLRGWTSVDIEG------HTYVTAMLQDLVPG-TDGYDYVTGTP-----------------DAFDATALGEAIRDVHTVLASTCETGTLAPGALATNLDRRLTGLVGRAPQLKEYEEKIRALYSTLAETSVPTQRIHGDLHLGQTLVDGDSWALIDFEGEPAASLEERRRPDSPLRDVAGMIRSFGYAAAASG-----------QDQSWADARTAELIAGYGEA-------DEAILRAYIADKCAYEVVYELENRPHMVHVPLDALASLV 4O7O Chain:B ((12-453)) -PWSDWLSRQRWYAGRNRELATVKPGVVVALRH-----N----LDLVLVDVTYTDGATERYQVLVGWDFEPASEYGTKAAIGVADDRTGFDALYDVAGPQFLLSLIVSSAVCGTSTGEVTFTREPDVELPFAAQPRVCDAEQSNTSVIFDRRAILKVFRRVSSGINPDIELNRVLTRAGNPHVARLLGAYQFGRPNRSPTDALAYALGMVTEYEANAAEGWAMATASVRDLFAEGDLYAHEVGGDFAGESYRLGEAVASVHATLADSLG-TAQATF-PVDRMLARLSSTVAVVPELREYAPTIEQQFQKLAAEAITVQRVHGDLHLGQVLRTPESWLLIDFEGEPGQPLDERRAPDSPLRDVAGVLRSFEYAAYGPLVDQATDKQLAARAREWVERNRAAFCDGYAVASGIDPRDSALLLGAYELDKAVYETGYETRHRPGWLPIPLRSIARLT

General information: TITO was launched using: RESULT: Template: 4O7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1601 -29065 -18.15 -77.71 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -18.15 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_4O7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4o7o-query.scw PDB file : Tito_Scwrl_4O7O.pdb: