Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------CTYSVYDSIGKMVVEDKDLPTNRRDYYTIGKKKYTFYFKENCDFDYSKPPLPRCWHVSGI--------------------------------------------------- 3HUH Chain:A ((20-143)) IIDRIDHLVLTVSDISTTIRFYEEVLGFSAVTFK---QNRKALIFGAQKINLHQQEMEFEPKASRPTPGSADLCFITSTPINDVVSEILQAGISIVEGPVERTGATGEIMSIYIRDPDGNLIEISQY

General information: TITO was launched using: RESULT: Template: 3HUH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 168 14608 86.95 256.27 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 86.95 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.307

(partial model without unconserved sides chains): PDB file : Tito_3HUH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3huh-query.scw PDB file : Tito_Scwrl_3HUH.pdb: