Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENHEIRDRVESVYGHS-VSGIRDVSSN-QKQTYVLDTSDGEVVYQRCESPQ-FRESFRCEVLLLEQMRDV---RTPVPKVLWRELENTP-EAVISSAPGREVSE-FYGEQNFEQAGDVVKQAGTTLSQIHEKKFNHSGKLSTQGGLELKATSWEDILAEIFGDYIHQLQGSRFEDSITDAVALFDNGLD-LV-DEHGSCLVHYDVAPDNVFWEN-GNISSVIDFGRAMSGDPAFDLAYAQVQFVLAFYDGREAEQLNRLLFGSYHTESLRGEWHKRMWFYRKLFIVEAMKSFESWTEGQGMSEEEKQESAEWNRDQLSQAAAELR 5IQI Chain:C ((13-279)) ---KAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLV----NNEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID----NKQNVLEEY-ILLRETIYNDLT-----DIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEI-----GTNFGEDILRMYGN-IDIEKAKEYQDIVEEYYPIETI------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1106 13548 12.25 52.92 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : 12.25 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: