Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDKKIIKIFKD-KLNEKVIKITDSSNGLAQIVKIVETKNKKYVFKNPKPG---DGIMIYRE-NFVCSNLKSKFIPKV-FFKTKN-----------YLIESFLEGTRPERNKP-EIFYYSLGKNIRKIHKVKMTGFGELQ--KNGKGQYKTAKQYIDNLIIENFPKFKNIKSFDKKILKYLE-KFIYENINCFDEKNSF---LLHFDLIPDNILM--QDNKLAGIIDFGDANCGPVEYDFGKLYLE----------------ISDKMFTKVLEGYGKKL-------EMKKIQYFVVLHLL-CILPYFYKV--------------NRKRYEKSVKLLRKFSKF--------------- 4OCV Chain:A ((24-378)) -TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNF-LSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADYLEGDVYFATKYPEHNLVRSRTQIKLVREMEQRADETRAIVADVMETT

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1198 42107 35.15 152.56 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 35.15 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.432

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: