Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVGETVDRIVADALE-AEVRSRYRPRSGSVAETVVCRLETGDGDGDEPPSRVVCKRGGASV-WTGDVIEPLVLEYMTAA-TDLPVPRVLASGSFADGSNDPLERWAIYEFREGANPGPRYHKL--------EPTVRRRLVADAGELLGRLHGTSALAFDRVGGLARGADDRSLRLREPDGWHA-VD--------PGWPLTRVPV-PL-AGDEDCRPVLTHGDYQPSNLLVDDDGAVTAVLDWGNAHVTHAEYALARAEARFVDVYAGRLPRAERKQLQELFRRTYANCAPLETGFDRRAPVYKLLWVAQSGANYLRIAREPRGRRQLRTQVRRLVE 5UXA Chain:A ((4-257)) -DIKQVIEIAKKHNLFLKEETIQFNESGLDFQAVFAQDN--------NGIDWVLRLPRREDVMPRTKVEKQALDLVNKYAISFQAPNWIIYT----------EELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPS---NKAAALDLVVHTP---EEARMSMKQRMDAVRAKFGVGENLWNRWQAWLNDDDMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAF------------GEEGLEALILAYKEIGGY---------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 913 15231 16.68 65.65 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 16.68 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.276

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: