Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLRNSDRTREEILTRYFPQ-Y-----RLIAPQAP-SGLGGASCIIEQG---------EQRLVLRQNHDPLASSSHFRRQFRALKR-LPADLVPKPRFYRQGWMAVEYLAGEIGNTLP-----ETAVLAAMLCHLHRQPRLGWR--VTLLPLLEQYWQQASADR-------RT----------PFWLAQLKRLRKTGEPQPLRLSPLHMDVHAGNIVHTSLGARLIDWEYAGDGDVALELAAVWSEDDA------------------A---RQRLITDYARVAHM--------NADALRRQVRRWRPWVVMLMAGWFEMRFQQSR-DKQFIALADDAWRQLQTKG 1NW1 Chain:B ((53-416)) ------KERAHMLCARFLGGAWKTVPLEHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPET-ESHLVAESVIFTLLSERHLGPKLYGIFSGGRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQLEVPIWKEPDYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHISLSKSPVTFCHNDLQEGNILLPK-RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELSPVGFGFADYGRDRLSLYF---

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1078 -47601 -44.16 -180.99 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -44.16 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: