Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHATSLPPELHQWVTRHLTGM------DTAAVTDASWPRGASRVWRVRTD---------HGEVFVKLYPST---EKYEREVQGCEHATRALAADEAPRLLASDPDRPAVVLSALPGRVVRGLPLEQAEEQRVHRLAGGLLRRWHDTPEPIGDREHQRIRASVTAQADEAAVILERLGD-QLAPA------ERNLVRDAAHDLPD-L-ARALPLAYRHGDYSPRNWLWDEAAGHHSLIDFEESAPGLAVEDLVWLCGAAWP-----------------TRPDLRDAFLTGYGRLLSS-----------TEQRALVLLTARLGVSYLSTGLTKAD-----TVLIDRGRTVLQHMVRAPR 1NW1 Chain:A ((53-414)) -------KERAHMLCARFLGGAWKTVPLEHLRISRIKGGM-SNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPETESHLVAESVIFTLLSER--HLG-PKLYGIFS--GGRLEEYIPSRPLSCHE---ISLAHMSTKIAKRVAKVHQLEVPIWKEPDYLC-EALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHISLSKSPVTFCHNDLQEGNILLPK---RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELSPVGFGFADYGRDRLSL------

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1134 -9924 -8.75 -36.49 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -8.75 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.340

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: