Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADSARYTFNMAGIIMHPGEKRVLLRAVQGGWMLPQVEWQADEYSSWKEVARFNREMQGLLDVCLSTVRCLGEDFERATMHFSRIYVMELHSSYDNLPADACWIDIEDLKEFMITPEEHLVWLEEWLAKRDEEERTPWYHPDWLAKAKVWIEQALAEPEAQPLVSIEQVGVAERGCLLRVQTKENAFYFKALPA------FFRHELTLLQHLARYRVGCVPALVAVDEREHRLLMHDFGSK-HLDQSLDLADWQEALRRYAILQIDHIQIDHIQIDHSAQVEQLEELGCPRLPLATLPDQLERLLTSEQSLLFDTPTALSLEQVRRLHDALPLIRSLCQELAGYGLPETLEHGDLHAQNIALSG-GDYLYFDWTDSCIAHPFFSLYPFLLDIEARWPEARIRLREAYLQPWERYAARAQLVEAFELAQRLAPLHLAMLYATLIIPHMKARWEMQHGVPLNLKVLLDRLHDAG 4R78 Chain:A ((41-252)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------SVEQL-GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLG-LDVKNYLFDI-EAGIKVNEYIESAITLDSTSIKTKFDKIAPILQTIH----------TSAKELRGE---F-APF-------EEIKKYESLIEEQ-------IPYANYESVR---NAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFT-----SQEEETFLSHYESDQ---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4R78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 804 -13230 -16.45 -64.85 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -16.45 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.182

(partial model without unconserved sides chains): PDB file : Tito_4R78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r78-query.scw PDB file : Tito_Scwrl_4R78.pdb: