Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELEPLLDSLAVLAGQEREVTLLPGGLTNANYRV----TTAT-----RDVVVRVASASSGLLSIDRDQEVHNSRAA----------AKAGVAPEVVEHRPGLLVVQFVP------DALTYGD--SDLAANLAGVADVVRRLHEGPAFA-GRFDMFDIQREYLAIMHTHRFRMPRGYLALAP----AAERMEAALRVHPEPRVPCHNDLLAANFL-------QDAQRLWIVDYEYSGMNEASFELGNIVQE------------------AHLTPEHLAELVTAYV-----------GHEDPVRTARAAL----WGIASAWAWTLWGTIQAGASDLDFDFWEWAMDKMDRAAQAFAEPGFDELLDTVGSPR 4CG8 Chain:A ((36-372)) ----------------EFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----------------EAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKY--LKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFD----------------------

General information: TITO was launched using: RESULT: Template: 4CG8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 804 -7195 -8.95 -29.98 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -8.95 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_4CG8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4cg8-query.scw PDB file : Tito_Scwrl_4CG8.pdb: