Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKIYEKYRDLITNPFDIKYKNIEIKEIISYPPAGNDVVECLCKIGKDARNVFIKIERSKVC-DFATEIKNLNYLKNNSYYSKIPNIYESGIYNNKQYIVLSKIEGERLSDILIKNDNLRKELLYKYGQELAIIHRIPISNQKIAKQRAIN-SYPNKTMYTTLSKENNIKEYIKFLEDNDFKKEL---------TTFIHGDFHYANVLWLNNDINGVIDWEYSGIGLREQDIA--WACTLRPGQKFMDTMEDIQTFLKGYSEKEKFDIEKLRWCLINSYCHFYLMNENNEDYKNKLLILLRNCMEEKI 4FEV Chain:A ((48-236)) ----------------------------------------------PNAPELFLKHGKGSVANDVTDEMVRLNWL---TAFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNC----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 601 3914 6.51 22.24 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 6.51 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.252

(partial model without unconserved sides chains): PDB file : Tito_4FEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4fev-query.scw PDB file : Tito_Scwrl_4FEV.pdb: