Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADRFIGSAVAVPASLDWLRATETGRIWLDKLPVLLETACE-HFGL-----SEIGSPFSGGNVSLVVPARH-D--------GEDVVLKLQFPDH---ECRHEAEALWRWNGKGA-VRLLKHAPELGALLLERCRPGQFLADDTE--TDRIGVVAGLLRLLTIPAGD--PF-TRLNDEAARWRDSLDHNWMAA---GKPC----EQYLVDAARKALQDLCLEKEAEEVLLHQDLHGHNILSSER------DTWLAIDPK-PLAGDPAFALSPIVRSFEFG----------------HSKAEARYRLDRLSEELEL----------------DRERARYWTIGQTMAWAFSSSYAERHFETVRWLLAEN 5FUT Chain:A ((4-350)) -----------------------PEPRTRRRAYLWCKEFLPGAWRGLREDEFH-ISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG---GAEAMVLESVMFAILAERSLGPKLYGIFPQ--GRLEQFI-PSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLES-TPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAK------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 941 3388 3.60 13.34 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 3.60 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: