Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRTTIDEAIARVDDWKGKEISYKPVSGGITNPNFKVDVE--GEHFFLKIPGAGT-DYINREVCHEANMIADESKAGPRVYYYFEDTGVEIFQWLDGYRQVTFGDVYDKDIFQSIFERIRDFHHLETKPLNL-------------KQSIFEQAWDMNARAKKGGY-ISP-------F-NDKMEYL---LSAIEKAL-AGSEELCPCHNDFWTNNLMYNEE-TNDLKIIDYEYASMNDPYVDLGLISAVNY---------FTEDMDVEMMKIYGGGKVD---ERAFARMKLNKIACDIKWAYWALYQAASSNVEFDYMNWYGQKMARLQHFWLDSRLDYWLDVAREKSTRIS 2QG7 Chain:E ((110-453)) -------------------SLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIAKKIYVFFTN--GRIEEFMDG-YALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGG-DSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSS-DFDFINYGMTRLTASCLPI--------------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1065 3495 3.28 13.24 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain E : 0.70 3D Compatibility (PKB) : 3.28 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: