Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRTTIDEAIARVDDWKGKEISYKPVSGGITNPNFKVDVEG---------EHFFLKIPGAGTDY--INREVCHEANMIADESKAGPRVYYYFEDTGVEIFQWLDGYRQVTFGDVYDKDIFQSIFERIRDFHHLETKPLNLKQSIFEQAWDMNARAKKGGY-----ISPF----NDKMEYLLSAIEKAL-AGSEELCPCHNDFWTNNLMYNEE-----TNDLKIIDYEYASMNDPYVDLGLISAVNYFT------------------EDMDVEMMKIYGGGKV--------------DERAFARMKLNKIACDIKWAYWALYQAASSNVEFDYMNWYGQKMARLQHFWLDSRLDYWLDVAREKSTRIS 5FUT Chain:A ((31-375)) -------------------EFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILAERSLGPKLYGIFPQ--GRLEQFIPS-RRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRK------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 928 5979 6.44 22.65 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 6.44 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: