Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRTTIDEAIARV-DDWKG---KEISYKPVSGGITNPNFKVDVEG----------EHFFLKIPGAGT-DYINREVCHEANMIADESKAGPRVYYYFEDTGVEIFQWLDGYRQVTFGDVYDKDIFQSIFERIRDFHHLETKPLNLK------QSIFEQAWDMNARAKKGGYI-------SPFNDKMEYLLSAIEKA--L-AGSEELCPCHNDFWTNNLMYNEETNDLKIIDYEYASMNDPYVDLGLISAVNYF------------------TEDMDVEMMKIYGGGKV-----D----ERAFARMKLNKIACDIKWAYWALYQAASSN--VEFDYMNWYGQKMARLQHFWLDSRLDYWLDVAREKSTRIS 3C5I Chain:D ((14-355)) --PLYIKKICLEKVPEWNHFTEDNLRVKQIL--LTNQLFEVGLKEETANNYNSIRTRVLFRIYGKHVDELYNTISEFEVYKTMSKYKIAPQLLNTF--NGGRIEEWLYG-DPLRIDDLKNPTILIGIANVLGKFHTLSR-KRHLPEHWDRTPCIFKMMEKWKNQLFKYKNIEKYNCDIHKYIKESDKFIKFMKVYSKSDNLANTIVFCHNDLQENNIINTN--KCLRLIDFEYSGFNFLATDIANFFIETSIDYSVSSYPFFEIDKKKYISYENRKLFITAYLSNYLDKVVPTPKLIDEILEAVEVQALGAHLLWGFWSIIRGYQTKSYNEFDFFLYAEQRLKMYDDQ---------------------

General information: TITO was launched using: RESULT: Template: 3C5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1092 20189 18.49 72.62 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.68 3D Compatibility (PKB) : 18.49 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_3C5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3c5i-query.scw PDB file : Tito_Scwrl_3C5I.pdb: