Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEFDSEDFSTSEEDEDYVPSGGEYSEDDVNELVKEDEVDGEEQTQKTQGKKRKAQSIPARKRRQGGLSLEEEEEEDANSESEGSSSEEEDDAAEQEKGIGSEDARKKKEDELWASFLNDVGPKSKVPPSTQVKKGEETEETSSSKLLVKAEELEKPKETEKVKITKVFDFAGEEVRVTKEVDATSKEAKSFFKQNEKEKPQANVPSALPSLPAGSGLKRSSGMSSLLGKIGAKKQKMSTLEKSKLDWESFKEEEGIGEELAIHNRGKEGYIERKAFLDRVDHRQFEIERDLRLSKMKP 5H0R Chain:F ((380-1161)) --------------------------------------------------------------------SLKQKYTNGVGSIAQSYLSSFTDEIAKRVNGI--------HHDEAWLNFLTTSSPGRKLTEIEKLEVGGDVAAWSNSRIVMQAVFAREYRTPERI----------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5H0R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 88 13520 153.64 155.40 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain F : 0.67 3D Compatibility (PKB) : 153.64 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.126

(partial model without unconserved sides chains): PDB file : Tito_5H0R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5h0r-query.scw PDB file : Tito_Scwrl_5H0R.pdb: