Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MNNSAFTFQTLHPDTIMDALFEQ------GIRVDS-GLTPLNSY-ENRVYQFQDEDR--------RRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEAD---NIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFEDATL---IPSGLKAAF--LKATDELIAAVTAHWRE-DFTVLRLHGDCHAGNILWRDGPMFVDLDDARNGPAIQDLWMLLNGDK---------------------AEQRMQLETIIEAYEEFS--EFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQDPPLQLTPMY 1NW1 Chain:A ((32-426)) GMKELLSTMDLDTDANTIPEL-KERAHMLCARFLGGAWKTVPLEHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYF-NPETESHLVAESVIFTLLSERHL--------GPKLYGIFSG--GRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQLEVPIWKEPDYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHISLSKSPVTFCHNDLQEGNILLPKRLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLL-----QVELSPVGFGFADYGRDRLSLYFKHKQL--LKNLA--

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1291 20724 16.05 67.50 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 16.05 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: