Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHPAAQPTNQTSSHSPASPSNTEPCEQQSLKQQLLKQQPLKEQSLKQQSREQQLHTWLNEVFPNQSLQHEKIPGDASFRSYHRLTVDNQHYIVMDAPPAKESVKEFIAVGNLMAGYVHVPKMIATDEQQGFIVLEDLGNTDFADVIAKDLTDAGELAKTARYYQQAMQEILAIQKIDINDAKAVIPSYDDALLRREMGLFSDWFLPYIGVKMTPDLETLWQDLQSDIIQQVIAQ-PQVVVHRDFHSRNLMVLSHSDELGVIDFQDAVIGAYTYDLASLLRDAYINYDENWVNTHLAHYHQLAQLDKSLAEFTVDFNIMSMQRHLKVLGIFVRLFERDGKDRYLVNLPKVFNDLLTCLKAISQWDKRPTFDKFADWMTAAAAPAFQQKIQG 1ZYL Chain:A ((4-328)) SAFTFQTLH------------------------------------------PDTIMDALFEHGIRVDSGLTPLNSYEN-RVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEA--DNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFE-DATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILW---RDGPMFVDLDDARNGPAVQDLWMLLNG-DKAEQRMQLETIIEAYEEFSEFDTAEIGL---IEPLRAMRLVYYLAWLMRRWADP-------AFPKNFPWLT------GEDYWLRQTATFIEQAKVLQEPPLQLTPMY

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1483 -100033 -67.45 -308.74 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -67.45 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.317

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: