Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIRPDIFSEIKQYIDIFH---IEPVRKGYSEDMKYLLTAKSGERFLLRITESDNTQI-LAGRKEMYSLMDTLAKYSSLVPRSRACWISHNAYSCIMIHEYIEGD-DGEESLHRYSQETQYEIGYRAGIELKKLHQLAAPATLSSWYTRKKQKHERYCDACNREHLAPEELDLEPIHRYIADNIHLMKNVSQTFQ------HDDYHPANLIIN-NGQLTGIIDFNRYDWGDPIHDF-YKLAFFSREISIPFSRGQIDGYFMGNVPSDFWKRYALYNAMSIVPSIVWFSRMASRKEFRDELKRAITLIRTLIDDHKGFTSAIPQWYQ 5IQI Chain:A ((19-266)) ----------IEHYFDNFKVDSIEIIGSGY-DSVAYLVNNE----YIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDEL--SILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEY--ILLRETIYNDLTDIEK--DYIESFMERL-NATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMY--GNIDIEKAKEY------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 967 22677 23.45 96.50 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 23.45 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: