Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELGNFQKSVDSDEIKTMAFEIIGEPVKAVSRICEQWGEAEDEVREYDVYVIETDAGKYTLKKTGS--------KEAQIYAQYLSK--GEFHVPQYVGMRQAEDADWICMKYVEGNDMRDMT---------DETTEKAAETLSKIQSYFWTPSMDKAPENEVEQRFVEYWKRVLRRASS-VADD-PILRKAYQMFLD--RQLTCPCTMSNGDFLQWNVIYDG-ENV-VMIDWGFGGMMPYPLDIARFLAHATETRSTFPFYMNDAQKELFLDRMYEALKTKISREQFNLDVKLATLNEYIEFVEAEEDEDGWYLEHAQALAEELLNL 5IQC Chain:A ((5-303)) ----YDDNATNVKAMKYLIEHYF-----DNFKVDSIE--IIGSGYDSVAYLV---NNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDN-KQNV-LEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEI-------GTNFGEDILRMYGNID--IEKAKEYQDIVEEYYPIETIVYGIKNI-KQEFIENGRKEIYKRTY--

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1217 19417 15.95 70.86 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 15.95 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: