Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDTDSLKNARFYATKTEPITDVDIVAEAPLERPELIKGKDAPGILTFARVH-REGGSDLYQLVVDKQ-G-------------------RDILGEEETARAAGKQLSNGYAV---GSGVLHKLTDPALPP-VETVRAV-GEQSNTSWIYNDRVIVKYFRRLTPTPNPEIELLEELTAAGCTHSAPLRGWTSVDIEG---HTYVTAMLQDLVPG-TDGYDYVTGTP-----------DAFDATALGEAIRDVHTVLASTCETGTLAPGAL-ATNLDRRLTGLVGRAPQLKEYEEKIRALYSTLAETSVPTQRIHGDLHLGQTLVDGDSWALIDFEGEPAASLEERRRPDSPLRDVAGMIRSFGYAAAASG---------------QDQSWADARTAELIAGYGEA-------DEAILRAYIADKCAYEVVYELENRPHMVHVPLDALASLV 4U98 Chain:A ((4-440)) -AFGDWIVHRRWYAGRSRELVSAEPAVVTPLRD-----D----LDHILLDVTYTDGTVERYQLVVRWADSPVAGFGEAATIGTALGPQGERIAYDALFDPDAARHLLRLVDASATVADLRFTREPGATLPLYAPPKVSSAEQSNTSVIFGKDAMLKVFRRVTPGINPDIELNRVLAQAGNRHVARLLGSFETSWAGPGTDRCALGMVTAFAANSAEGWDMATASAREMFADVVGSDFADESYRLGNAVASVHATLAEALG---TSTEPFPVDTVLARLQSAARSAPELAGRAAAVEERYRRLDGRAITVQRVHGDLHLGQVLRTPDDWLLIDFEGEPGQPLDERRRPDSPLRDVAGVLRSFEYAAYQKLVELAPEQDADGRLADRARNWVDRNSAAFCAGYAAVAGDDPRRDGDVLAAYELDKAVYEAAYEARFRPSWLPIPMRSIDRIL

General information: TITO was launched using: RESULT: Template: 4U98.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1724 -36561 -21.21 -98.02 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -21.21 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_4U98.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4u98-query.scw PDB file : Tito_Scwrl_4U98.pdb: