Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVGETVDRIVAD-ALEA--EVRSRY--RPRSGSVAETVVCRLETGDGDGDEPPSRVVCKRGGASV------WTGDVIEPLVLEYMTAATDLPVPRVLASGSFADGSNDPLERWAIYEFREGANPGPRYH---------KLEPTVRRRLVADAGELLGRLHGTSALAFDRVG--GLARGADDRSLRLR---EPDGWHA-VD----PGWPLTRV-PVP-LAGD---EDCRPVLTHGDYQPSNLLVDDDGAVTAVLDWGNAHVTHAEYALARAEARFVDVYAGRLP-RAERK---QLQELFRRTYANCAPLETGFDRRAPVYKLLWVAQSGANYLRIAREPRGRRQLRTQVRRLVE 3ATT Chain:A ((2-318)) -LPAVISRWLSSVLPGGAAPEVTVESGVDSTGMSSETIILTARWQQDGR-SIQQKLVARVAPAAEDVPVFPTYRLDHQFEVIRLVGELTDVPVPRVRWIETTGD---VLGTPFFLMDYVEGVVPPDVMPYTFGDNWFADAPAERQRQLQDATVAALATLHSIP---NAQNTFSFLT-----DTTLHRHFNWVRSWYDFAVEGIGRSPLLERTFEWLQSHWPDDAAAREPVLLWGDARVGNVLYR-DFQPVAVLDWEMVALGPRELDVAWMIFAHRVFQELAGLATLPGLPEVMREDDVRATYQALTGVEL---GDLHWFYVYSGVMWAC------------------------

General information: TITO was launched using: RESULT: Template: 3ATT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1177 4853 4.12 17.78 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 4.12 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.303

(partial model without unconserved sides chains): PDB file : Tito_3ATT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3att-query.scw PDB file : Tito_Scwrl_3ATT.pdb: