Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVGETVDRIVADALEAEVRSRYRPRSGSVAETVVCRLETGDGDGDEPPSRVVCKRGGASVWTGDVIEPLVLEYMTAATDLPVPRVLASGSFADGSNDPLERWAIYEFREGANPGPRYHKLEPTVRRRLVADAGELLGRLHGTSALAFDRVGGLARGADDRSLRLREPDGWH----AVD---PGWPLTRV-PVP-LAGDEDCRPVLTHGDYQPSNLLVDDD-GAVTAVLDWGNAHVTHAEYALARAEARFVDVYAGRLPRAERKQLQELFRRTYANCAPLETGFDRRAPVYKLLWVAQSGANYLRIAREPRGRRQLRTQVRRLVE 4H05 Chain:B ((21-265)) -----------------------EDGASGAGVYRLRGG---------GRELFVKVAALGAGVGLLGEAERLVWLAE-VGIPVPRVVEGGGDE------RVAWLVTEAVPGRPASARW---PREQRLDVAVALAGLARSLHALD---WERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGERLLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRELAHEED----PWFGPECSAAFLREYGRGWDGAV-SEEKLAFYRLLDE-----------------------------

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1058 -26967 -25.49 -114.75 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -25.49 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.276

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: