Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAANPPRPSRSLQGKVAIVTGAGSRGETIGNGRAIALLLAEDGAKVVCVDREEALAQRTAELVLAENEGASALAVAADVSSAEECARVVDAALRLFGRVDVLVNGVGVLG--PPGTAVDVDAARWARGLEVNVTSMMLMAKYAVPAMRDNEPEAGVRGSIVNLGSVAGLRGGTPSLLYPTSKCAVVNMTRAMAAHHAKDGVRVNCVCPGMLYTPMV---YDAEGGMSAEVREARRRRSLLQTEGNAWDCAGPVRFLAGGEARWITGTVLTVDAGATCALAVSMPDGE 2CFC Chain:D ((3-248)) ---------------RVAIVTGASS-----GNGLAIATRFLARGDRVAALDLSAETLEETAR-THWHAYADKVLRVRADVADEGDVNAAIAATMEQFGAIDVLVNNAGITGNSEAGVLHTTPVEQFDKVMAVNVRGIFLGCRAVLPHMLLQGA-----GVIVNIASVASLVAFPGRSAYTTSKGAVLQLTKSVAVDYAGSGIRCNAVCPGMIETPMTQWRLD-----QPELRDQVLARIPQKEIGTAAQVADAVMFLAGEDATYVNGAALVMDGAYT-----------

General information: TITO was launched using: RESULT: Template: 2CFC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1342 -107116 -79.82 -444.46 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.74 3D Compatibility (PKB) : -79.82 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_2CFC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2cfc-query.scw PDB file : Tito_Scwrl_2CFC.pdb: