Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNTYFELSDDFENIIKNTLNDKQIISIKQNPTGWTNIVYEVETNEGNFFFRFPRDEFWSRTIVKDYEFAKYIYGKTNFDTVKLELFYDN----DRPFSVHKKISGIPLAEVMNDL-SSDEVKTISDEIAKFMYQLHCLDFKESK-----IFSINNIGLNLTDFL--DELLNVHVDKNDMNFWI----PTAIHSDDICLVHGDLNSSNVLLDE-NRHVAAIIDFGFGGFGNKYNDIGRIIGR---CPTSFKNEIINSYEQY-SNSKLDSKLLDTNINTWTDIDNSYINYMRTIGIYK 3N4U Chain:A ((6-263)) --------FDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEI---NRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEK----------------------------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 967 -28574 -29.55 -120.57 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -29.55 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.329

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: