Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVENMGRDSMWQAISTLLRDWHTEDAEIELKTELPGGEIHSA--------WHLR----FGGKDYFVKC---DERELLPIFTAESDQLELLSRSKTVRVPQVFAVGSDRDYSFVVMEYLPPRPLDAHNAFLLGQQLAHLHQWSDQPQFGLDFDNDLSTTPQPNAWQRRWSVFFAEQRIGWQLELAAEKG-LHFGDI---DTLVDMVQQRLANHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDRECDLAMLPMHP-EQPPQIYDGYQSVSPLPSGFLDRQPIYQLYTLLNRAILFGGQHLVTAQQALDDVLMEKMR 3F7W Chain:A ((10-277)) -------------------------------TELTGREVAAVAERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQV-AGWDDRTLAMEWVDERPPTPEAAER---FGHQLAAMHL-AGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDALDHLAGDPEP-PARIHGDLWNGNVLWQDDGAVVIDPAAHGGHREADLAMLALFGLPYLDRVRDAYNEVAPLAEGWRARIPLHQLHPLLVHVCLFGAAYRTT--------------

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1232 -6974 -5.66 -28.12 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -5.66 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: