Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAPAQSEQMRRLAGLLGVKLDPFSTGGESLTFGITGTELLVTIPTEP-GEQDVQRQAELTRRVADRISIPVPEIVRVVAEAEAVVVR-----RLPGVPLIAVPTPQRAALTRSVALA-VGTVLAELHTWDRDGYADLAPVDEYSPEEWRAETAELVSDLTPLLTTDQR-------DDVRRFLGRPAPRPAGRHVLSHNDLGIEHILVS--PDAVTGVIDWNDAAITDPAYDFGLLLRD---LGPEALRTALARYAHQIGGDPELLERASYYAVCALLEDLAFGHETGRVEYVAKSLHGWRWTFQAAG 4N57 Chain:A ((51-316)) --------------------------GNDCIAYEIN-RDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLD------FREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHK--DIPTVLEKYRMKEKYWSFEKIIYGKEYGYMDWYEEGLNEIR-------

General information: TITO was launched using: RESULT: Template: 4N57.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1076 -167 -0.16 -0.68 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -0.16 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_4N57.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4n57-query.scw PDB file : Tito_Scwrl_4N57.pdb: