Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEVREYGKKSQPKIRYSQFDAEDIRKELLERYGMEMPVECRLFDYGMNDIYRIKTKKETYFLRISLAGLHTKKDYEEEMHILNILIQNGIKAAAPVKMRGMPGRQEDYVWELHAPEGIRYAMLFTEAPNSPSDDKESSACN----LGSMLAGIHNISDR--------------EKFQVSRPPIDMCQLVEKPLSNIRPFLADREQDFSFLKESAEKLAGYIKGKLPETAPVYGFCHGDVHSGNVFFAGDKP----TLFDFDCMGYGWRSYDICVYAWNETFLDENYIEGSVWKKYLEGYESVRPLSAEEKETIPAFAALRQLWLMGLHADVMDRNAGCCWYQDDYFDSQIKMFRLWLDRCSL 6EF6 Chain:A ((30-309)) ------------------------IAQQALTHYDVSDNASLRLLNLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDS-DVTVPTVVPARDGRRVVTVDPAD---VPRHVVHF-EMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLT-RAQDLLH-----RKLEEYGSG--PDR---YGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAV---SFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFL-------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1125 -47420 -42.15 -185.96 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -42.15 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: