Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATLRDGR-WIERRPRRPAVTAQLRREASFLPWLAPALPLRVPVPRI----LVDQPGHEFVSTHELVPGEPLCAF-----TSANGRRLGRFLRALHSRSSDDAVRHGLL--PASGAAEDLANDVNLFRRTVLPLVPAGWRPDAQAVLDRVGTFP-FDTVVHGDLGPEHVLTCD-DVITGVIDFGDARCGDAAMDFSWVLHGTPSVFGEAAAEEYGVTPAVRERARVWRRLSPWYDVTRGLGTGDADMVRDGLDGLVNRLRKP 5IGJ Chain:A ((40-263)) IATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREF--VVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHL----MVFGEEGLAKLLLTYEA-AGGRVWPRLA-------------------------------

General information: TITO was launched using: RESULT: Template: 5IGJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 823 1458 1.77 6.94 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 1.77 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_5IGJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igj-query.scw PDB file : Tito_Scwrl_5IGJ.pdb: