Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLPRRPDADPAALLAGLDRSLAGLGLTEVGEGFDNIAYAVRSPSGARLLLRV---SKVADPVE-RARVLTKDVALLEFANRHSSLTTNEVIAADA-HAGALLLTEVTGRTADQIRPTDRVAFAATMATFLTRLHTAPV---VEAALVTEMDPRSAAQWL---VDTATAWAAVADRTPVTLRDRVDEFLVGPAPSDVRRITFCHNDLGDEHVVLSEDGGHVRGVIDWSDAVLGDPARDLGLLTLDFGPGIVDALIDDYHGPTGADFRGRVLWFAATAGVEGLAWRLANARPWQVTADRLHDVLAVLA 5IGJ Chain:A ((46-256)) ---------------------------------------------GRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRT-PTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVV-----VHGDLYVGHVLI-DNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGG--------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 774 -10839 -14.00 -54.19 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -14.00 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.178

(partial model without unconserved sides chains): PDB file : Tito_5IGJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igj-query.scw PDB file : Tito_Scwrl_5IGJ.pdb: