Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSYEFN-VTQLRDLPFGDKNIKIITEKKGGWDSRFFILDGKVAVKVPRNITVQHSIIKEKIVTDLLRKELPLPIPDMNLAEIDSIDGNKIIIVIYEMLPGIELQNIHII---GENKIKTSKSLGLFLQKLHNI-----PDELAQILRSGISNVNNERNETDLELLNSVLEYFEEMRNKTNVSSYIEIMRTSMGSLKQMGTKIVPC--HGDFMSNNILYNEIEGKISGIIDWGDFSFRNPIYDFAGL---TYEFGEEFLDSMLAAYASNTSPDFREKIVKLSRFVPFITVYFSRHMKKKIRFEKKYIKIELLESGRNQILHVR 4N57 Chain:A ((22-317)) -RTYTFDQVEKAIEQLYPDFTINTIQISGKGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPS-ETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKI----NEDNKKIKKLLSRELK-GPQMKKVDDF--YRDILEN------EIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSF----------EKIIYGKEYGYMDWYEEGLNEIRS--

General information: TITO was launched using: RESULT: Template: 4N57.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1253 47869 38.20 169.75 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 38.20 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.360

(partial model without unconserved sides chains): PDB file : Tito_4N57.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4n57-query.scw PDB file : Tito_Scwrl_4N57.pdb: