Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence AGISNTNPPGGAPRPRQQCFLSADLGPDVMGQPTLLE-------ATPFVNDMFDFHGYRCQMGKDCSHVRCRGFPAAFKMTAR 5B66 Chain:E ((36-73)) ------------------LFVSTGLAYDVFGTPRPDSYYAQEQRSIPLVTDRFEAK---------------------------

General information: TITO was launched using: RESULT: Template: 5B66.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 30 -1795 -59.82 -57.89 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain E : 0.66 3D Compatibility (PKB) : -59.82 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.134

(partial model without unconserved sides chains): PDB file : Tito_5B66.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5b66-query.scw PDB file : Tito_Scwrl_5B66.pdb: