Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence KNCKFNVLNGSGINVANGCCDVTKKQVTVS----------PFTVSCTESCGLAIVSGG 6E14 Chain:H ((167-220)) ----WNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYLSG

General information: TITO was launched using: RESULT: Template: 6E14.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 48 -5801 -120.84 -131.83 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain H : 0.78 3D Compatibility (PKB) : -120.84 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.342

(partial model without unconserved sides chains): PDB file : Tito_6E14.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6e14-query.scw PDB file : Tito_Scwrl_6E14.pdb: