Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----KGCKFKILNENRVTVAEGCCRRDEKFVVVGNKAGGSLRVKCGISC 1GPS Chain:? ((1-47)) KICRRRSAGFKGPCMSNKNCAQVC--QQEGW-GGGNCDGPFRRCKCIRQC

General information: TITO was launched using: RESULT: Template: 1GPS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 140 2569 18.35 61.17 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 18.35 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.198

(partial model without unconserved sides chains): PDB file : Tito_1GPS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1gps-query.scw PDB file : Tito_Scwrl_1GPS.pdb: