Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLRNSDRTREEILTRYFPQYRLIAPQAP-SGLGGASCIIEQG--EQRLVLRQNHDPLASSSHFRRQFRALKR-LPADLVPKPRFYRQGWMAVEYLAGEIGNTLP-----ETAVLAAMLCHLHRQPRLGWR---------------VTLLPLLEQYWQQASADR----------------RTPFWLAQLKRLRKTGEPQPLRLSPLHMDVHAGNIVHTS-LGARLIDWEYAGDGDVALELAAVWSEDD-----------AA-RQRLITDYARVAH-MNADALRRQVRRWRPWVVMLMAGWFEMRFQQSRDKQFIALADDAWRQLQTKG 2QG7 Chain:D ((110-450)) ----------------------SLEFQIINGG-TNILIKVKDMSKQAKYLIRLYGPKTD---NREREKKISCILYNKNIAKKIYVFFTNGRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTASC---

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 956 -7762 -8.12 -31.68 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain D : 0.63 3D Compatibility (PKB) : -8.12 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: