Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAG-HDFFVKCDEREL-LPGFTAEADQLELLSRSKTVTVPKVWAVGADR-DYSF----LVMDYLPPRPLDA--------HSAFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAAEKGIAFGNIDAIVEHIQQRLAS---HQPQPSLLHGDLWSGNCALGP-D-GPYIFDP-ACYWGDRECDLAMLPLH--TEQPPQIYDGYQSVSPLP-----ADFLERQPVYQLYTLLNRARLFGGQHLVIAQQSLDRLLAA 5IGI Chain:A ((11-294)) ---QLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPIS-AAVDAG--MLI--RTPTQARQKVADDVDR----VRREF-VV-------NDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAK---------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1135 -46122 -40.64 -180.87 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -40.64 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: