Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPFDEVRRTVTGHGLELVRAH-------PRRDRLHLDLRGPDRQRVIGQWIDDATEATR------VADA--TPGRVERLGSRLVLQHD----GADRRLPALADLVA-----GGAELVVHRPERRAVV-------RYQRGDGYV--YTKVVR--PSRTAELVRRVRAAAAVPGLSAPEVTQWDEAGGTIVLTTLPGQTLHDLLTGVVPAVVATEVGRAVRTLHSAATAPDGTTTHDLAAEVDATRALLDLARTHRALQPREALVVDRRIAAAAATIAALARPAAPSLLHRDLHDKQLLVDGASIGMLDVDTLGLGDPALDLGNLLAHLDLRVRQGWTQRETAAAVEQGVLDGYVPDDRTRAATAGYRALAAARLEALYAFRPGDLPGGGQPAK 5JK4 Chain:A ((2-373)) --------TAVTGGGASLPADLYKGSADSILPANFSYAVTGSGTGKKAFLENNSALFSTTGTVHFAGSDSVLSSTELNTYNSTYNVSGDANRYGALVQIPSVATSVTIPFNKAGSAVDLSVTQICGIFSGKINNWSQLAGLGRTGAIQVVYRGESSGTSELLTRFLTSACQPADVSG--TNLKLANGVPAFSVQS--TFANLFTTVPSNFVAAP-ATGGSALYNAVYAVDGRVGYVGPDVIPSLTDATKVAKVKS-FSPDEVSVQATLETAAPPTGAAAENPANWVPVFGNP-SAGYPIAGYTNFVFGQCYKNATVGANVRGFLTRHYGSTVVNGVEQGPNDDAIRA---HKFIP--LTKAWRDAVRARFATATNAGAVNNPSTCSGIGRPL-

General information: TITO was launched using: RESULT: Template: 5JK4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1955 21981 11.24 65.22 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 11.24 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.155

(partial model without unconserved sides chains): PDB file : Tito_5JK4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5jk4-query.scw PDB file : Tito_Scwrl_5JK4.pdb: